ELISA-HRP底物TMB ONE/TMB ONE (& prestained)

TMB（3，3'，5，5'-四甲基联苯胺）是辣根过氧化物酶（HRP）的发色底物。 在HRP对过氧化氢进行酶促降解过程中，TMB产生深蓝色，添加终止溶液产生清晰的黄色，并在450 nm处吸收。 与OPD（邻苯二胺二氯化物）相比，该底物可提供更高的信号并在ELISA中具有更高的灵敏度。

TMB ONE characteristics are:

• “The Gold Standard” used across the industry
• Incredibly stable and robust
• Aqueous based – no organic solvents (REACH Annex XVII compliant)
• Low lot-to-lot variation

For traceable pipetting, we recommend using pink colored TMB ONE Prestained (cat. no. 4430). The pink indicator disappears after addition of the stop solution.

The product is available in bulk quantities and as customized solutions.

Specifications：

Available sizes: 100 mL, 500 mL, 1 L , bulk                      Format:Ready-to-use (RTU)
Storage: 2-8⁰C in the dark                                             Stability: 4 years
Development time: 5-30 minutes                                     Stop solution: 0.2 M H₂SO₄